Desmoplakin (DSP) (1672 -1684) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/4000 (Detection limit).  Western blot: Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1 µg/ml).   Immunohistochemistry on Paraffin Sections: 3-6 µg/ml. In paraffin embedded Human Heart shows staining of the junctons between cardiomuscular fibres.
Reactivity	Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide from the internal region of Human DSP / Desmoplakin (NP_004406.2).
Specificity	This antibody is expected to recognize reported isoform I (NP_004406.2) only.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	desmoplakin
Database Link	Entrez Gene 1832 Human UniProt ID P15924
Background	Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in this gene are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.
Synonyms	DP, DSP, 250/210 kDa paraneoplastic pemphigus antigen
Reference Data

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