Vimentin (VIM) Chicken Polyclonal Antibody

CAT#: AP31825PU-N

Vimentin (VIM) chicken polyclonal antibody, Aff - Purified



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CNY 7,688.00


货期*
5周

规格
    • 300 ug

Product images

Specifications

Product Data
Applications IF, IHC, WB
Recommend Dilution Western Blot.
Immunocytochemistry.
Immunohistochemistry.
Recommended Dilutions:
1/2000-1/5000 for Western Blots.
1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells.
Quality Control: Each of the three antibodies was analyzed by Immunohistochemistry (at a dilution of 1/2000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
Reactivity Human, Mouse
Host Chicken
Clonality Polyclonal
Immunogen Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Vimentin gene product, but are shared between the Human (NP_003371) and Mouse (NP_035831) sequences.
Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Specificity Recognizes Vimentin.
Formulation 10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative.
State: Aff - Purified
State: Liquid purified (filter sterilized) IgY fraction.
Concentration lot specific
Purification Affinity Chromatography using a peptide column.
Conjugation Unconjugated
Storage Condition Store the antibody undiluted in the dark at 2-8°C.
Gene Name vimentin
Background Human vimentin is a 53,521 dalton protein (466 amino acids) expressed in many cell types of mesodermal origin, and is one of the intermediate filament types that form the cytoskeleton. In the embryonic nervous system, however, vimentin is expressed by migrating neural crest cells forming the PNS, and by neural stem cells of the CNS. In some forms of glioblastoma, vimenin is reexpressed by the
tumor cells and serves as a general indicator of their undifferentiated state.
Synonyms VIM
Reference Data
Protein Families ES Cell Differentiation/IPS
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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