Synaptotagmin 1 (SYT1) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot. Immunocytochemistry. Immunohistochemistry. Recommended Dilutions: 1/2000-1/5000 for Western Blots. 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Quality Control: Each of the four antibodies was analyzed by Immunohistochemistry (at a dilution of 1/2000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
Reactivity	Human, Mouse
Host	Chicken
Clonality	Polyclonal
Immunogen	Four different antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (NP_033332) and Human (NP_005630) gene products. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.4 mg/ml. Finally, equal volumes of each of the four affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized. Antibodies were affinity-purified and mixed in equal concentrations.
Specificity	Recognizes Synaptotagmin-1.
Isotype	IgY
Formulation	10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified (filter sterilized) IgY fraction.
Concentration	lot specific
Purification	Affinity Chromatography using a peptide column.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted in the dark at 2-8°C.
Gene Name	synaptotagmin 1
Database Link	Entrez Gene 20979 MouseEntrez Gene 6857 Human UniProt ID P21579
Background	Human Synaptotagmin-1 is a 47,442 dalton protein (422 amino acids) associated with pre-synaptic terminals of the PNS and CNS. Synaptotagmin-1 binds calcium and initiates the fusion of vesicles with the pre-synaptic membrane. This protein is also believed to be the target of proteolytic activity associated with Botulinum neurotoxin A.
Synonyms	SYT1, SVP65, SYT, Synaptotagmin1, Synaptotagmin I, SytI, p65
Reference Data
Protein Families	Secreted Protein, Transmembrane

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