Amyloid beta (N-term) Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC
Recommend Dilution	Immunocytochemistry. Immunohistochemistry. Recommended Dilutions: 1/2000-1/5000. Quality Control: Antibodies were analyzed by Immunohistochemistry (at a concentration of 3 µg/ml) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) or HRP-labeled Goat anti-Chicken IgY (1/2000 dilution, Cat.-No AP31795HR-N) as the secondary reagent.
Reactivity	Human, Mouse
Host	Chicken
Clonality	Polyclonal
Immunogen	Hens were immunized with a synthetic peptide KLH conjugated corresponding to DAE FRH DSG YEV HHQ KL, residues 1-17 of the Amyloid beta-peptide (Amyloid Precursor Protein residues #672-688). After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks.
Specificity	Recognizes Beta-Amyloid Peptide (N-term).
Isotype	IgY
Formulation	10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified (filter sterilized) IgY fraction.
Concentration	lot specific
Purification	Affinity Chromatography using a peptide column.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted in the dark at 2-8°C.
Background	Beta-Amyloid peptide is a 40- or 42-amino acid fragment of the Human Beta Amyloid Precursor Protein (770 amino acids) produced by the proteolytic actions of Beta and Gamma-secretases. Both forms of Beta-amyloid peptide are rather insoluble and tend to self-aggregate into distinctive extracellular “plaques.” These plaques are evident in brains from patients with Alzheimer’s disease, as well as in brains from individuals with a history of traumatic head injuries. In the case of Alzheimer’s disease, it has been suggested that these extracellular Beta-amyloid peptide plaques are themselves cytotoxic (rather than simply being markers of brain pathology), and are responsible for the dendritic pruning and other neurodegenerative changes seen.
Reference Data

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