Junctional Adhesion Molecule 1 (F11R) (1st extracell. dom.) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IHC, WB
Recommend Dilution	Flow cytometry: The typical starting working dilution is 1:50. Immunohistochemistry on paraffin sections: The typical starting working dilution is 1:50. Western blot: The typical starting working dilution is 1:50. Not useful for Immunoassays.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Extracellular domain of full-length human JAM-A
Specificity	The antibody reacts with the 12 kDa extracellular domain 1 of the human JAM-A protein.
Isotype	IgG
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein A
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	F11 receptor
Database Link	Entrez Gene 50848 Human UniProt ID Q9Y624
Background	Together with JAM-C (JAM-2) and JAM-B (VE-JAM or JAM-3), JAM-A belongs to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAMs are important for a variety of cellular processes, including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. JAM-A is expressed by endothelial and epithelial cells, platelets, neutrophils, monocytes, lymphocytes and erythrocytes. Like all other JAMs, JAM-A play an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-A also plays an important role in leukocyte transmigration. Leukocyte transmigration can be blocked by antibodies and by soluble JAM-A/Fc fusion proteins. Homophilic JAM-A interactions between leukocytes and the endothelium but also heterophilic interactions of JAM-A with the b2-integrin leukocyte function-associated antigen-1 (LFA-1) are considered to actively guide leukocytes during transmigration. Several studies imply a role of JAM-A in the initiation of atherosclerosis, since JAM-A is upregulated on early atherosclerotic endothelium and adhesion of activated platelets on activated endothelium is mediated by homophilic interactions of JAM-A.
Synonyms	JAM-A, Platelet F11 receptor, F11R, JCAM, PAM1
Reference Data

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