CEACAM1 (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, WB
Recommend Dilution	Western blot: 1-5 μg/ml. ELISA: 1-5 µg/ml. FACS: 2-20 μg/ml.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure recombinant Human soluble CEACAM-1 (N-term)
Specificity	This antibody will detect recombinant Human soluble CEACAM-1. It shows cross-reactivity with several other CEACAM familiy members. There is no cross-reactivity with Human CEACAM-8.
Formulation	PBS pH 7.4 State: Aff - Purified State: Lyophilized purified IgG fraction Preservative: None
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Protein A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	carcinoembryonic antigen related cell adhesion molecule 1
Database Link	Entrez Gene 634 Human UniProt ID P13688
Background	Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM1; also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I trans-membrane proteins (CEACAM1, 3-4) and GPI-linked molecules (CEACAM5-8). There is no CEACAM2 in human. The gene for human CEACAM1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type followed by three C2-type Ig-like domains. It shows considerable glycosylation (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1-3). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V-type Ig-like domain (aa's 35-142). The three soluble forms also contain the first two C2-type Ig like domains (aa's 145 317), with differences coming in the third C2-type Ig- like domain (6). The seven transmembrane isoforms are highly divergent. Full-length mouse and rat CEACAM1 are approximately 57% aa identical to human CEACAM1; in the V-type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium.
Synonyms	BGP, BGP1, Biliary glycoprotein 1
Reference Data
Protein Families	Druggable Genome, Transmembrane

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