Semaphorin 3E (SEMA3E) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: Detection Limit: 1/128000. Western blot: Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0.03 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2kDa according to NP_036563.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: 4-6 µg/ml. In paraffin embedded Human Prostate shows membranous staining of secretory cells in the gland.
Reactivity	Canine, Human, Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_036563.1.
Specificity	Recognizes Semaphorin 3E (internal).
Formulation	Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	semaphorin 3E
Database Link	Entrez Gene 9723 Human UniProt ID O15041
Synonyms	Semaphorin-3E, SEMAH, KIAA0331
Reference Data

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