MMP2 (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: At 1μg/ml with the appropriate system to detect MMP2 in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect MMP2 in formalin fixed and paraffin embedded tissues. Immunohistochemistry on frozen sections: At 1-2 μg/ml to detect MMP2 in formalin or acetone fixed tissues. Immunocytochemistry: At 1-2μg/ml to detect MMP2 in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide corresponding to a sequence at the C-terminal of human MMP2
Specificity	This antibody detects MMP-2 (C-term).
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2ml of distilled water will yield a concentration of 500μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	matrix metallopeptidase 2
Database Link	Entrez Gene 17390 MouseEntrez Gene 81686 RatEntrez Gene 4313 Human UniProt ID P08253
Background	Matrix metalloproteinase-2 (MMP2) is a Type IV collagenase, 72-kD, which is also known as gelatinase1 and is a member of a group of secreted zinc metalloproteases. The MMP2 gene is 17 kb long with 13 exons varying in size from 110 to 901 bp and 12 introns ranging from 175 to 4,350 bp 3, located within a region of human chromosome 16q13. In addition,The extra exons encode the amino acids of the fibronectin-like domain which has so far been found in only the 72-and 92-kDa type IV collagenase. MMP2,which has a critical role in the binding of progelatinase A and TIMP4 via the C-terminal hemopexin-like domain (C domain), is functionally associated on the surface of angiogenic blood vessels. NOT only is a likely effector of endometrial menstrual breakdown4, MMP2 is also effector and regulator of the inflammatory response. Moreover, MMP2 could be helpful in diagnosing Takayasu arteritis.
Synonyms	MMP2, CLG4A, 72 kDa gelatinase, Matrix metalloproteinase-2, Gelatinase A, TBE-1
Reference Data
Protein Families	Druggable Genome, Protease
Protein Pathways	Bladder cancer, GnRH signaling pathway, Leukocyte transendothelial migration, Pathways in cancer

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