ATP5PF Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: At 0.5-1μg/ml with the appropriate system to detect ATP5J in cells and tissues. Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect ATP5J in formalin fixed and paraffin embedded tissues.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide mapping at the middle region of human mature ATP5J (CF6)
Specificity	This antibody detects ATPase subunit F6 (mid region).
Isotype	IgG
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2ml of distilled water will yield a concentration of 500μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6
Database Link	Entrez Gene 11957 MouseEntrez Gene 94271 RatEntrez Gene 522 Human UniProt ID P18859
Background	ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 (ATP5J) is a multisubunit membrane-bound enzyme complex consisting of an F0 segment embedded in the membrane and an F1 segment attached to the F0. It is also a component of mitochondrial ATP synthase which is required for the interactions of the catalytic and proton-translocating segments. Human ATP5J shares 72% sequence identity with rat ATP5J.This import signal peptide is rich in basic amino acids, devoid of acidic amino acids, and amphiphilic, which allows it to be water-soluble yet capable of passage through the phospholipid membrane bilayers. Moreover, it is circulating and functions as an endogenous vasoconstrictor by inhibiting cytosolic phospholipase A2.
Synonyms	ATP5J, ATP5A, ATPM, F1F0 ATPase subunit F6, Complex V subunit F6
Reference Data
Protein Pathways	Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease

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