Lamin B1 (LMNB1) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, IP, WB
Recommend Dilution	Western blot: At 1-2μg/ml with the appropriate system to detect Lamin B in cells and tissues. Immunohistochemistry on paraffin sections: At 0.5-1μg/ml to detect Lamin B in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunohistochemistry in frozen sections: At 0.5-1μg/ml to detect Lamin B in formalin or acetone fixed tissues. Immunocytochemistry: At 0.5-1μg/ml to detect Lamin B in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required. Immunoprecipitation.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a sequence at the C-terminal of human Lamin B
Specificity	This antibody detects Lamin Beta at C-term. No cross reactivity with other proteins.
Formulation	5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	0.2 ml of distilled water will yield a concentration of 0.5 mg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	lamin B1
Database Link	Entrez Gene 16906 MouseEntrez Gene 116685 RatEntrez Gene 4001 Human UniProt ID P20700
Background	Lamins are the major components of the nuclear lamina which underlies the nuclear envelope of eukaryotic cells. lamin B is a structural component of the long-sought-after spindle matrix that promotes microtubule assembly and organization in mitosis. Inspection of the deduced amino acid sequence of lamin B revealed the presence in coil 1B of the alpha-helical domain of a leucine heptad repeat region.Lamin B assembled into a matrix-like network in mitosis through a process that depended on the presence of the guanosine triphosphate-bound form of the small guanosine triphosphatase Ran.
Synonyms	Lamin B1/B2, LMN2, LMNB, Lamin B1, Lamin B2
Reference Data

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