HIF-1 alpha (HIF1A) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, IP, WB
Recommend Dilution	Western blot: At 1-2 μg/ml with the appropriate system to detect HIF-1α in cells and tissues. Immunohistochemistry on Paraffin Section: At 1-2 μg/ml to detect HIF-1α in formalin fixed and paraffin embedded tissues. Boiling the sections is required.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A peptide mapping at the N-terminus of HIF-1α of Human origin, different from the related Rat and Mouse sequences by two sequences.
Specificity	This antibody detects HIF1A / HIF1 alpha.
Formulation	50% Glycerol, 0.9 mg NaCl, 0.2 mg Na2HPO4 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	1.2% Sodium Acetate or neutral PBS. If 0.5 ml of PBS is used, the antibody concentration will be 200 μg/ml.
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	hypoxia inducible factor 1 alpha subunit
Database Link	Entrez Gene 15251 MouseEntrez Gene 29560 RatEntrez Gene 3091 Human UniProt ID Q16665
Background	HIF-1α (Hypoxia-inducible factor 1α,HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1α transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1alpha is over expressed during carcinogenesis, myocardial infarction and wound healing.It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers,resulting in the activation of genes essential for cell survival. HIF-1α regulates the survival and function in the inflammatory microenvironment directly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.
Synonyms	HIF-1 alpha, Hypoxia-inducible factor 1 alpha, ARNT-interacting protein, Member of PAS protein 1, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78
Reference Data
Protein Families	Transcription Factors
Protein Pathways	mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma

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