EIF4EBP2 (99-120) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry on Paraffin Sections: 10 µg/ml. Western Blot: 4 µg/ml.
Reactivity	Human, Bovine, Canine, Mouse, Porcine, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	EIF4EBP2 antibody was raised against human PHAS-II synthetic peptide (residues 99-120) was synthesized and the peptide coupled to KLH.
Specificity	Detects 17 and 20 kD proteins, corresponding to the apparent molecular mass of PHAS-II and its phosphorylated state on SDS-PAGE immunoblots.
Formulation	BBS, pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA) State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	eukaryotic translation initiation factor 4E binding protein 2
Database Link	Entrez Gene 13688 MouseEntrez Gene 361845 RatEntrez Gene 1979 Human UniProt ID Q13542
Background	PHAS-II, also known as eIF4E-binding protein II (eIF4E-BPII), is a member of a family of proteins which regulate initiation. PHAS-I and -II were found to have overlapping but different patterns of expression in tissues. PHAS-II shows the highest expression in liver and kidney where very little PHAS-I is found. The PHAS proteins regulate translation initiation by binding to the inhibitory protein eIF- 4E and blocking translation by preventing access of eIF-4G to the 5' cap of the mRNA. Both PHAS proteins are phosphorylated in response to insulin or growth factors such as EGF, PDGF and IGF-1. Phosphorylation in the appropriate site(s) promotes dissociation of PHAS/eIF-4E complexes which allows eIF-4E to bind eIF-4G(p220), thereby increasing the amount of eIF-4F complex and the rate of translation initiation. Regulation of the two protein differ because PHAS-II, unlike PHAS-I is readily phosphorylated by PKA in vitro. However increasing cAMP in cells promotes dephosphorylation of both PHAS-I and PHAS-II. Pharmacological and genetic evidence indicates that the mTOR/p70S6K pathway is involved in the control of PHAS-I and -II suggesting that these proteins may be mediators of the effects of this pathway on protein synthesis and cell proliferation.
Synonyms	EIF4EBP2, eIF4E-binding protein 2
Reference Data
Protein Families	Transcription Factors

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