Thermolysin Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice. Recommended Working Dilutions: Vary widely, but may be between 1/10,000 and 1/100,000.
Reactivity	Bacillus sp.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Thermolysin isolated and purified from Bacillus thermoproteolyticus rokko. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	Affinity purified antibodies of polyclonal rabbit antiserum to Thermolysin from Bacillus thermoproteolyticus rokko. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Isotype	IgG
Formulation	PBS, pH 7.2 stabilized with 0.5 % Dextran without preservatives and foreign proteins State: Aff - Purified State: Lyophilized purified hyperimmune IgG fraction
Reconstitution Method	Restore by adding 0.5 ml of sterile distilled water
Concentration	2.0 mg/ml (after reconstitution)
Purification	Solid Phase Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P00800
Synonyms	Thermostable Neutral Proteinase, npr
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.