Phospholipase C / PLC Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	Can be used for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques). Working dilutions: vary widely, but may be between 1/1000 - 1/20000.
Reactivity	Bacillus sp.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Phospholipase C isolated and purified from Bacillus cereus. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to phospholipase C from Bacillus cereus. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Isotype	IgG
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized purified IgG fraction. Molar radio: Biotin/IgG ~5.4
Reconstitution Method	Restore by adding 1,0 ml of sterile distilled water.
Concentration	lot specific
Purification	Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and DEAE-Chromatography.
Conjugation	Biotin
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P09598
Synonyms	Cereolysin A
Reference Data

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