Nuclease P1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice. Working dilutions in non-precipitating antibody-binding techniques: 1/100-1/500.
Reactivity	Penicillium
Host	Rabbit
Clonality	Polyclonal
Immunogen	Nuclease P1 isolated and purified from Penicillium citrinum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	IgG fraction to Nuclease P1 from Penicillium citrinum. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
Isotype	IgG
Formulation	PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized purified Hyperimmune IgG fraction
Reconstitution Method	Restore by adding 1.0 ml sterile distilled water. Prepare working dilutions by adding sterile phosphate buffered saline (PBS, ph 7.2) and preferably use the same day.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P24289
Synonyms	Endonuclease P1, Deoxyribonuclease P1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.