Hyaluronidase Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IP, R, WB
Recommend Dilution	This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/2,000-1/10,000).
Reactivity	Sheep
Host	Rabbit
Clonality	Polyclonal
Immunogen	Hyaluronidase isolated and purified from Sheep testes. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Isotype	IgG
Formulation	PBS, pH 7.2 without preservatives and foreign proteins. Label: Biotin State: Lyophilized IgG fraction. Molar radio: Biotin/IgG: ~1.0
Reconstitution Method	Restore by adding 1.0 ml sterile distilled water.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Background	Hyaluronidase degrades hyaluronic acid, which is an important structural proteoglycan found in basement membranes and also extracellular matrices. There are six members of the hyaluronidase family. Hyaluronidase PH20 is a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane and plays a role in sperm penetration through the the hyaluronic acid-rich cumulus cell layer surrounding the oocyte. Abnormal expression of this gene has been implicated in degradation of basement membranes leading to tumor invasion and metastasis.
Synonyms	HYAL1, HYAL2, LUCA1, LUCA-1, HYAL-1, Hyal-2, Hyaluronoglucosaminidase, Hyaluronidase
Reference Data

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