SNAP23 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.1-0.3 µg/ml. This antibody detects a band of ~26kDa Human Placenta lysates. Immunohistochemistry on Paraffin Sections: 3.75 µg/ml.
Reactivity	Bovine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_003816.2 and NP_570710.1.
Specificity	This antibody is expected to recognize both reported isoforms (NP_003816.2 and NP_570710.1).
Formulation	Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	synaptosome associated protein 23kDa
Database Link	Entrez Gene 20619 MouseEntrez Gene 64630 RatEntrez Gene 8773 Human UniProt ID O00161
Background	Synaptosomal-associated proteins (SNAPs) are cytosolic proteins that play a key role in in the process of membrane fusion in intracellular vesicle trafficking. In eukaryotic cells, the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex is critical to membrane docking and fusion and is believed to impart some degree of specificity between vesicle SNARE (v-SNARE) and target organelle SNARE (t-SNARE). In neurons and neuroendocrine cells, the SNARE complex consists of the integral membrane proteins VAMP (vesicle-associated membrane protein), syntaxin and SNAP-25. In non-neuronal tissue, a SNAP-25 homolog, SNAP-23, functionally replaces SNAP-25 in the SNARE complex. Studies show that VAMP, syntaxin and SNAP-23 are required for SNARE function and that this complex exists as a heterotrimer of the three proteins. In insulin signalling pathways, studies suggest that the translocation of GLUT4 in adipocytes requires that functional SNAP-23 be present in SNARE complexes.
Synonyms	SNAP-23
Note	Calculated Molecular Weight: 23.4kDa (NP_003816.2
Reference Data
Protein Families	Druggable Genome
Protein Pathways	SNARE interactions in vesicular transport

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