PAK2 Rabbit Polyclonal Antibody

CAT#: AP21121PU-N

PAK2 rabbit polyclonal antibody, Aff - Purified



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CNY 1,999.00

CNY 4,640.00


货期*
2周

规格
    • 100 ug

Product images

Specifications

Product Data
Applications IHC, WB
Recommend Dilution Western blot: 1/500 - 1/1000.
Immunohistochemistry on paraffin sections: 1/50 - 1/200.
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Specificity This antibody detects endogenous levels of PAKγ protein.
(region surrounding Arg186)
Formulation Phosphate buffered saline (PBS), pH 7.2.
State: Aff - Purified
State: Liquid purified Ig fraction
Preservative: 0.05% sodium azide
Concentration 1.0 mg/ml
Purification Affinity chromatography (> 95% (by SDS-PAGE)
Conjugation Unconjugated
Storage Condition Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Predicted Protein Size ~ 58 kDa
Gene Name p21 (RAC1) activated kinase 2
Background Three isoforms of serine/threonine kinases, designated 7PAK p68, 8PAK p65 and 9PAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The 7, 8, and 9 PAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on 9 PAK, including Ser 19, Ser 141 Thr 402, phosphorylation of Ser 141 and Thr 402 is correlated with 9 PAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
One such putative substrate is Mek kinase, an upstream effector of Mek4, which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP dependent manner with Rac 1 and Cdc 42, they do not interact with Rho.
Synonyms PAK 2, PAK-2, Gamma-PAK, PAK gamma, PAK65, p21-activated kinase 2, p58
Reference Data
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Axon guidance, ErbB signaling pathway, Focal adhesion, MAPK signaling pathway, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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