SDF1 (CXCL12) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to amino acids 50-100 of Human SDF-1.
Specificity	This antibody detects endogenous levels of SDF-1 protein. (region surrounding Met93)
Formulation	Phosphate buffered saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~10.0 kDa
Gene Name	C-X-C motif chemokine ligand 12
Database Link	Entrez Gene 20315 MouseEntrez Gene 24772 RatEntrez Gene 6387 Human UniProt ID P48061
Background	SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes, but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven-pass, transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (also designated LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major co-receptor for T-tropic HIV-1, and SDF-1 has been shown to inhibit HIV-1 infection. Six human SDF-1 isoforms exist due to alternative splicing of CXCL12, the gene encoding SDF-1.
Synonyms	Stromal cell-derived factor 1, SDF1A, SDF1B, IRH, hIRH, PBSF
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	Axon guidance, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Leukocyte transendothelial migration

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