14-3-3 zeta (YWHAZ) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/500 - 1/1000. Immunohistochemistry on Paraffin Sections: 1/50 - 1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 49-100 of Human 14-3-3 ζ.
Specificity	This antibody detects endogenous levels of 14-3-3 ζ protein. (region surrounding Ala79)
Formulation	Phosphate Buffered Saline (PBS), pH~7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~28 kDa
Gene Name	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
Database Link	Entrez Gene 22631 MouseEntrez Gene 25578 RatEntrez Gene 7534 Human UniProt ID P63104
Background	14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, theta, epsilon, zeta, sigma and nu. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzyme activity etc.
Synonyms	YWHAZ, KCIP-1
Reference Data
Protein Pathways	Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis, Pathogenic Escherichia coli infection

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