KSR2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 662-724 of Human Ksr2.
Specificity	This antibody detects endogenous levels of Kinase suppressor of Ras 2. (region surrounding Ile694)
Formulation	PBS, pH 7.2 State: Aff - Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE) Preservative: 0.05% Sodium Azide
Concentration	1.0 mg/ml
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 108 kDa
Gene Name	kinase suppressor of ras 2
Database Link	Entrez Gene 283455 Human UniProt ID Q6VAB6
Background	Kinase suppressor of Ras (KSR) and MAP kinase kinase kinase (MEKK3) are integral members of the MAP kinase pathway. Ksr is a conserved protein that positively regulates Ras signaling and may function as a scaffold for Raf, MEK and ERK. There are two types of Ksr proteins: Ksr-1 and Ksr-2. These two are individually necessary for a few specific Ras-dependent processes, but they are required together for most aspects of Ras-mediated signaling. Ksr-2 plays a key role in Ras-mediated signaling during germline meiotic progression and functions redundantly with Ksr-1 during the development of the excretory system pathway, hermaphrodite vulva and male spicules. Ksr-2 also functions as a negative regulator of the MEKK3-mediated activation of the MAP kinase pathways (specifically ERK and JNK) and of the NFκB pathways, and it simultaneously inhibits MEKK3-mediated IL-8 production.
Synonyms	KSR2, KSR-2, hKSR2, hKSR-2
Reference Data

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