Aurora A (AURKA) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 1/500-1/1000. Immunohistochemistry on paraffin sections.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to amino acids 251-300 of Human AURKA.
Specificity	This antibody antibody detects endogenous levels of of AurA protein.
Formulation	Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~ 48 kDa
Gene Name	aurora kinase A
Database Link	Entrez Gene 20878 MouseEntrez Gene 261730 RatEntrez Gene 6790 Human UniProt ID O14965
Background	Aurora 2/AIK, a cell cycle-regulated serine/threonine protein kinase, is overexpressed in many tumor cell lines. Phosphorylation of threonine 288, which is within the activation loop of the kinase, results in a significant increase in its enzymatic activity. Aurora 2/AIK is regulated by proteasome-dependent degradation, and phosphorylation on Thr288 may target it for degradation during mitosis. Thus, phosphorylation of Aurora 2/AIK at Thr288 is important for both kinase activity and stability.
Synonyms	AURKA, AIK1, ARK1, AURA, BTAK, STK15, STK6, Aurora/IPL1-related kinase 1, AURORA2
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways	Oocyte meiosis

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.