Synaptotagmin 1 (SYT1) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Peptide ELISA: Detection Limit: 1/16000. Western Blot: 0.3-1 µg/ml. Approx. 65kDa band observed in Human Brain (hippocampus) and Mouse and Rat Brain lysates (calculated MW of 47.6kDa according to NP_005630.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Matthew et al, J Cell Biol. 1981 Oct;91(1):257-69.; PMID: 7298720). Immunohistochemistry on Paraffin Sections: 4-6 µg/ml. In paraffin embedded Human Brain shows vesicular staining in neuronal cell and axon.
Reactivity	Bovine, Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_005630.1.
Specificity	This antibody recognizes Synaptotagmin I.
Formulation	Tris saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	synaptotagmin 1
Database Link	Entrez Gene 6857 Human UniProt ID P21579
Synonyms	SYT1, SVP65, SYT, Synaptotagmin1, Synaptotagmin I, SytI, p65
Reference Data

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