Cytokeratin 10 (KRT10) Guinea Pig Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot. Immunofluorescence Microscopy on Frozen Sections. Immunohistochemistry on Paraffin Sections (After Microwave treatment). Working Dilution: 1/100-1/200 for Immunohistochemistry. Incubation Time: 1h at RT. Positive Control: Human suprabasal keratinocytes of epidermis; squamous cell carcinoma.
Reactivity	Human, Mouse, Rat
Host	Guinea Pig
Clonality	Polyclonal
Immunogen	Synthetic peptide of Human Keratin K10 (formerly also designated Cytokeratin 10; C-GS VGE SSS KGP RY), coupled to KLH
Specificity	MW 56,5000 (pI 5.3) intermediate filament polypeptide, keratin K10, detected by Immunohistochemistry in the suprabasal layers of Human epidermis (basal cells are negative). Useful for the recognition of keratinizing cells in squamous cell carcinoma of epidermis, lung, bladder, cervix, esophagus etc. Completely negative on non-stratified epithelia.
Formulation	State: Serum State: Liquid Serum with 0.09% Sodium Azide and 0.5% BSA
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	keratin 10
Database Link	Entrez Gene 16661 MouseEntrez Gene 450225 RatEntrez Gene 3858 Human UniProt ID P13645
Background	Cytokeratin 10 is a heterotetramer of two type I and two type II keratins. Cytokeratin 10 is generally associated with keratin 1. It is seen in all suprabasal cell layers including stratum corneum. A number of alleles are known that mainly differ in the Gly-rich region (positions 490-560). Defects in cytokeratin 10 are a cause of epidermolytic hyperkeratosis (EHK), also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. Defects in cytokeratin 10 are also a cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens.
Synonyms	Cytokeratin-10, CK10, CK-10, Keratin-10, KRT10, KPP, K10
Reference Data

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