Biotin (F(ab')2 fragment) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Western Blot: 1/200 - 1/1,000. Dot Blot. ELISA: 1/2,000 - 1/10,000. Immunohistochemistry: 1/100 - 1/500.
Host	Goat
Clonality	Polyclonal
Immunogen	Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Specificity	This is a Peroxidase conjugated F(ab)2 fragment of Anti-Biotin.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) and 0.01% (w/v) Sodium Azide Label: HRP State: Lyophilized F(ab)2 fragment Label: Horseradish Peroxidase (Molecular Weight 40,000 daltons)
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water
Concentration	lot specific
Purification	Immunoaffinity chromatography
Conjugation	HRP
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Synonyms	Vitamin B7, Vitamin H
Reference Data

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