FITC F(ab)2 Fragment Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1:2,000 - 1:12,000. Western blot: 1:500 - 1:2,500. Dot blot. Immunohistochemistry on frozen sections: 1:200 - 1:1,000.
Host	Goat
Clonality	Polyclonal
Immunogen	Fluorescein conjugated to Goat IgG
Specificity	This antibody detects Fluorescein F(ab')2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Isotype	IgG
Formulation	0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free; 0.01% (w/v) Sodium Azide Label: AP State: Liquid sterile filtered Ig fraction Label: Alkaline Phosphatase (Calf Intestine); Molecular Weight 140,000 daltons
Concentration	lot specific
Purification	Prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation	AP
Storage Condition	Store the antibody prior and following reconstitution (undiluted) at 2 - 8 °C. Do not freeze!
Synonyms	Fluorescein Isothiocyanate
Reference Data

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