GST-Tag Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/20,000. Western Blot: 1/2,000-1/5,000. Immunohistochemistry: 1/500-1/2,000.
Host	Goat
Clonality	Polyclonal
Immunogen	Full length GST isolated from Schistosoma japonicum.
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Isotype	IgG
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative. Label: HRP State: Lyophilized purified Ig fraction. Label: Horseradish Peroxidase
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Immunoaffinity Chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer.
Conjugation	HRP
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P08515
Background	GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
Synonyms	Glutathione S-transferase Tag, GST26-Tag
Reference Data

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