Protein A Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, IHC
Recommend Dilution	Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Flow cytometry: 1:2,000 - 1:10,000. Immunoflourescence: 1:500 - 1:2,500.
Host	Goat
Clonality	Polyclonal
Immunogen	Protein A [Staphylococcus aureus]
Specificity	This antibody detects Protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus], anti-Goat IgG and anti-Goat Serum.
Isotype	IgG
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 as buffer, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer, 0.01% (w/v) Sodium Azide as preservative Label: Texas Red State: Lyophilized Ig fraction Label: (TM) Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption emission: 596 nm / 620 nm Molar radio: 1.5 moles Texas Red(TM) per mole of Goat IgG
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Texas Red
Storage Condition	Store vial at 2-8° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for two weeks at 2-8° C as an undiluted liquid. Dilute only prior to immediate use.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.