Protein A Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. The product has been assayed against 1.0 µg of Protein A in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Host	Goat
Clonality	Polyclonal
Immunogen	Protein A [Staphylococcus aureus]
Specificity	This antibody detects Protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus]. Cross reactivity against Protein A from other sources is unknown.
Isotype	IgG
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 as buffer, 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer, 0.01% (w/v) Sodium Azide as preservative Label: Biotin State: Lyophilized Ig fraction Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Store vial at 2-8° C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for two weeks at 2-8° C as an undiluted liquid. Dilute only prior to immediate use.
Reference Data

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