XAF1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Immunohistochemistry on Paraffin Sections: 5 µg/ml. Western Blot: 0.5 - 2 µg/ml.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic Peptide corresponding to amino acids at the C-terminus of human XAF-1.
Specificity	This antibody recognises XIAP-Associated Factor 1.
Formulation	Phosphate Buffered Saline PBS containing 0.02% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C to -70°C for longer. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Gene Name	XIAP associated factor 1
Database Link	Entrez Gene 54739 Human UniProt ID Q6GPH4
Background	XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP’s ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-a2 and IFN-b can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.
Synonyms	XIAPAF1, BIRC4BP
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.