POLK Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA: 1/2000 - 1/10000. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 5 µg/ml. Western Blot: 1/500 - 1/3000.
Reactivity	Human, Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to Amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative. State: Aff - Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted (in aliquots) at -20°C. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Gene Name	polymerase (DNA) kappa
Database Link	Entrez Gene 51426 Human UniProt ID Q9UBT6
Background	POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair. POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity. POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
Synonyms	POLK, DINB1, DINB protein, DINP
Reference Data

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