Bcl2 Binding component 3 (BBC3) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Western Blot: 1 - 2 µg/ml.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide from the C-terminal region of Human PUMA
Specificity	This antibody recognizes a synthetic peptide corresponding to 14 amino acids near the carboxy terminus of PUMA-alpha. This sequence is identical between alpha and beta forms of the PUMA proteins.
Formulation	PBS State: Aff - Purified State: Liquid purified Ig fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -70°C for longer. Avoid repeated freezing and thawing.
Gene Name	BCL2 binding component 3
Database Link	Entrez Gene 27113 Human UniProt ID Q96PG8
Background	Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Synonyms	BBC3, JFY-1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.