DYKDDDDK Epitope Tag Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: 1:9000 - 1:450000. Western Blot: 1:2000 - 1:10000.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK. Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
Specificity	This antibody is specific for the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins. This antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays. In Western blotting of bacterial extracts this antibody has been shown not to cross-react with any endogenous proteins.
Isotype	IgG
Formulation	0.02M Potassium phosphate with 0.15M Sodium chloride pH7.2 containing 0.09% Sodium Azide (NaN3) State: Purified State: Liquid purified IgG
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Synonyms	FLAG Epitope Tag, ECS Epitope Tag, FLAG-tag, ECS-tag, D-tag
Reference Data

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