FITC Sheep Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections.
Host	Sheep
Clonality	Polyclonal
Immunogen	Fluorescein isothiocyanate (Isomer 1).
Specificity	This antibody recognises Fluorescein isothiocyanate. This preparation has been cross-absorbed against Mouse, Human and Rat serum proteins.
Formulation	Borate buffered saline without preservatives. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at -20°C. Avoid repeated freezing and thawing.
Background	FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
Synonyms	Fluorescein Isothiocyanate
Reference Data

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