Phosphothreonine Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	ELISA: 0.5 μg/ml (Ref.5) Western blot: 2 μg/ml (Ref.5). 2 μg/mL of this antibody was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium. Immunoprecipitation: 10 μg/mg sample.
Host	Rabbit
Clonality	Polyclonal
Immunogen	Phosphothreonine conjugated to KLH
Specificity	This antibody recognizes proteins phosphorylated on Threonine residues (Species Independent). Does not cross-react with Phosphotyrosine.
Isotype	IgG
Formulation	PBS State: Purified State: Liquid purified Ig fraction Stabilizer: 50% Glycerol Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
Note	Centrifuge vial before opening.
Reference Data

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