Orf1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Suitable for Western Blotting under Reducing conditions (0.2-1 μg/ml).
Reactivity	Neisseria meningitidis
Host	Rabbit
Clonality	Polyclonal
Immunogen	Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
Specificity	The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.
Formulation	PBS, pH 7.4 with 15 mM sodium azide as preservative. State: Aff - Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE).
Concentration	lot specific
Purification	Protein-A affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.
Reference Data

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