DNA PKcs (PRKDC) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1x TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide sequence around amino acids 2607~2611 (V-E-T-Q-A) derived from Human DNA PK.
Specificity	This antibody detects endogenous levels of total DNA PKcs protein.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol. State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Immunoaffinity Chromatography using epitope-specific peptide.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	protein kinase, DNA-activated, catalytic polypeptide
Database Link	Entrez Gene 5591 Human UniProt ID P78527
Background	DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer.
Synonyms	DNPK1, p460, HYRC, HYRC1
Note	Molecular Weight: 450 kDa
Reference Data

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