ERK1 (MAPK3) pThr202 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1/500-1/1000. Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Syntetic phosphopeptide derived from Human p44/42 MAP Kinase around the phosphorylation site of Threonine 202 (F-L-Tp-E-Y).
Specificity	This antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at Threonine 202.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl and 50% Glycerol State: Aff - Purified State: Liquid purified IgG fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	42, 44 kDa
Gene Name	mitogen-activated protein kinase 3
Database Link	Entrez Gene 5595 Human UniProt ID P27361
Background	Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.
Synonyms	ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK
Reference Data

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