ZAP70 pTyr319 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, WB
Recommend Dilution	Western Blot: 1/500 - 1/1000.; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence: 1/100 - 1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from human Zap-70 around the phosphorylation site of Tyrosine 319 (S-P-Yp-S-D).
Specificity	This antibody detects endogenous levels of VEGFR2 only when phosphorylated at Tyrosine 1214.
Formulation	PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	70kd
Gene Name	zeta chain of T cell receptor associated protein kinase 70kDa
Database Link	Entrez Gene 7535 Human UniProt ID P43403
Background	Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Synonyms	ZAP-70, ZAP 70, SRK
Reference Data

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