MEF2A pThr312 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA: 1/20000-1/40000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. Immunoprecipitation: 1/50-1/200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Specificity	This antibody detects endogenous levels of MEF2A protein only when phosphorylated at Thr312.
Formulation	Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Concentration	1.0 mg/ml
Purification	Affinity Chromatography using epitope-specific immunogen.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	myocyte enhancer factor 2A
Database Link	Entrez Gene 4205 Human UniProt ID Q02078
Background	MEF-2 is a muscle-specific DNA binding protein that recognizes an A+T-rich sequence [CTA (A/T)4 TAG] localized in the control regions of numerous muscle-specific genes. MEF-2 belongs to the MADS (MCM1, Agamous, Deficiens and serum-response factor) box family of transcription factors. The MEF-2 proteins arise from several alternatively spliced isoforms of the MEF-2 gene. MEF-2 expression is ubiquitous, but appears to be preferential in skeletal and cardiac muscle cells. Phosphorylation of different MEF-2C isoforms affects their expression pattern and transactivation function. Big MAP kinase 1 (BMK1) enhances the transactivation activity of MEF-2C by phosphorylating Ser 387. Serum is a potent stimulator of BMK1-induced MEF-2C phosphorylation. p38 MAPK can phosphorylate MEF-2C at positions Ser 387, Thr 293 and Thr 300. Phosphorylation of MEF-2C by either p38 MAPK or ERK5/BMK1 is necessary for Smad-MEF-2 signaling cooperativity.
Synonyms	MEF2, Myocyte-specific enhancer factor 2A
Note	Molecular Weight: ~55.0 kDa
Reference Data

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