Timd4 Rat Monoclonal Antibody [Clone ID: RMT4-54]

Specifications

Product Data
Clone Name	RMT4-54
Applications	FC, IF, IHC
Recommend Dilution	Flow cytometry: 1/25-1/200, use 10µl of this suggested working dilution to label 10e6 cells in 100µl. Immunofluorescence. Immunohistochemistry on Paraffin sections. Positive control: Mouse spleen. For functional assays Cat.-No. AM60019LE-N is recommended.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse TIMD4 (amino acids 1-288), coupled with the Fc portion of mouse IgG2a
Specificity	Clone RMT4-54 specifically recognises mouse Tim-4. Studies have shown that clone RMT4-54 significantly inhibits the efficient phagocytosis of apoptotic cells by PRMs (peritoneal resident Mac1+ cells), but not PEMs (peritoneal exudate Mac1+ cells) [Nakayama, 2009].
Isotype	IgG2a
Formulation	PBS containing 0.09% Sodium azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	T cell immunoglobulin and mucin domain containing 4
Database Link	Entrez Gene 276891 Mouse UniProt ID Q6U7R4
Background	TIMD4 / TIM-4 (T-cell immunoglobulin mucin 4), a transmembrane glycoprotein and member of the immunoglobulin superfamily, exclusively expressed by antigen-presenting cells, which acts as the natural ligand of TIM-1, involved in T-cell expansion and cytokine production. TIMD4 is reported to be crucial for the phagocytosis of apoptotic cells by peritoneal macrophages, and studies have shown that interaction between TIM-1 on Th2 cells and TIM-4 on DCs, enhances Th2 cell function. The TIMD4 gene locus is associated with predisposition to asthma in both mice and humans and may be considered as a candidate disease gene for asthma.
Synonyms	TIMD-4, TIM4, TIM-4, T-cell membrane protein 4
Reference Data

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