GCLM Mouse Monoclonal Antibody [Clone ID: AT2D12]

Specifications

Product Data
Clone Name	AT2D12
Applications	ELISA, FC, ICC/IF, IF, WB
Recommend Dilution	ELISA. Western blot: 1/3000 (using HeLa cells (40 µg), resolved by SDS-PAGE). Flow cytometry: 2-5 µg in 1x106 cells (in HeLa cell line). Immunofluorescence/Immnunocytochemistry: 1/100 (in HeLa cell line).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human GCLM (1-274aa) purified from E. coli
Isotype	IgG1, kappa
Formulation	Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Concentration	1mg/ml (determined by BCA assay)
Purification	By protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Gene Name	glutamate-cysteine ligase modifier subunit
Database Link	NP_002052 Entrez Gene 2730 Human UniProt ID P48507
Background	GCLM (Glutamate-cysteine ligase, modifier subunit), also known as gamma-glutamylcysteine synthetase, is first rate limiting enzyme of glutathione synthesis. GCLM is heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. GCLM deficiency has been implicated in some forms of hemolytic anemia. Also, GCLM is a molecular target for amelioration of cisplatin restance in lung cancer.
Synonyms	Glutamate-cysteine ligase modifier subunit, Glutamate-cysteine ligase, modifier subunit, GLCLR
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Glutathione metabolism, Metabolic pathways

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