CD5 Mouse Monoclonal Antibody [Clone ID: MCD5]

Specifications

Product Data
Clone Name	MCD5
Applications	FC, IF, IHC
Recommend Dilution	MCD5 is used in the identification and localization of T cells in tissue and the diagnosis of T cell lymphomas and of B cell lymphocytic lymphomas of CLL types. - Flow cytometry: for analysis of blood and bone marrow samples. Used in flow cytometry for the enumeration of T cells and CD5 positive B cells in peripheral blood (see "Protocols" below). - Immunofluorescence / Immunohistochemistry using cytospots or frozen tissue sections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	MCD5 recognizes a 67 kD antigen on human T cells. Other species not tested.
Formulation	0.01 M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2% BSA, 0.09% sodium azide State: Aff - Purified State: Liquid purified protein
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	CD5 molecule
Database Link	Entrez Gene 921 Human UniProt ID P06127
Background	CD5 appears to be a relatively late marker during B cell differentiation. CD5 expression is thought to be absent on surface Ig negative B-lineage cells but appears on IgM+ cells in both fetal liver and bone marrow. It co-precipitates with the T cell receptor and, in particular with Lck. A role for CD5 in signal transduction is postulated based on stimulatory effects of CD5 monoclonal antibodies. CD5 antigen is phosphorylated on tyrosine residues on T cell activation. There is evidence that CD5 plays a role in thymocyte selection, as well as a role in cell-cell recognition. Recently CD5 on B cells has been shown to be an endogenous ligand selective for B-cell surface IgFR (framework region) sequences. Interaction of surface Ig with CD5, other endogenous antigens or (in mucosal sites) exogenous superantigens can provide B cells with continual stimulation and might prevent their elimination from the immune system. In addition, B cell superantigens, e.g. Staphylococcus aureus Cowan strain 1, may contribute to the pathogenesis of autoimmune diseases and malignancies.
Synonyms	CD5, LEU1
Note	1. Conjugates with brighter fluorochromes, like PE and APC, will have a greater separation than those with dyes like FITC. When populations overlap, the percentage of positive cells using a selected marker can be affected by the choice of fluorescent label. 2. Use of monoclonal antibodies in patient treatment can interfere with antigen target recognition by this reagent. This should be taken into account when samples are analyzed from patients treated in this fashion. 3. Reagent data performance is based on EDTA-treated blood. Reagent performance can be affected by the use of other anticoagulants. Protocol: Flow cytometry method for use with purified monoclonal antibodies 1. Add 100 µl of EDTA-treated blood (i.e. approx. 10e6 leukocytes) to a 5 ml reagent tube. The content of one tube is sufficient to perform one test. 2. Add to each tube 10 µl of purified monoclonal antibody. (Appropriate mouse Ig isotype control samples should always be included in any labeling study). Vortex the tube to ensure thorough mixing of antibody and cells. 3. Incubate the tube for 15 minutes at room temperature in the dark. 4. Wash the labeled cells by adding 2 ml of PBS containing 0.001% (v/v) Heparin, vortexing and centrifuging (2 min 1000 x g) and discard the supernatant. 5. Add 50 µl of appropriate dilution of F(ab)2 Rabbit Anti Mouse IgG fluorescent conjugate (e.g.FITC or R-PE) in PBS containing 0.001% (v/v) Heparin to the tube. It is recommended that the tube is protected from light. 6. Mix by vortexing and incubate for 15 minutes at room temperature in the dark. 7. Add 100 µl of a lyse reagent and mix immediately. 8. Incubate for 10 minutes at room temperature in the dark. 9. Add 2 ml of demineralized water and incubate for 10 minutes in the dark. 10. Centrifuge the labeled cell suspension for 2 minutes at 1000 x g. 11. Remove the supernatant and resuspend the cells in 200 µl of PBS. 12. Analyze by flow cytometry within four hours (alternatively, the cells may be fixed by 0.05% of formaline in buffered saline for analysis the next day. Some antigens are readily destroyed upon fixation and this should be taken into account when using this alternative).
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Hematopoietic cell lineage

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.