CDC20 Mouse Monoclonal Antibody [Clone ID: AR12]

Specifications

Product Data
Clone Name	AR12
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/ml. Western Blotting: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: Ramos or HeLa cells; Tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Urea-denatured His6 Cdc20 Human recombinant protein.
Specificity	This antibody reacts with Human Cdc20 (55 kDa). Other species not tested. Cellular Localization: Cytoplasmic.
Isotype	IgG1
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	55 kDa
Gene Name	cell division cycle 20
Database Link	Entrez Gene 991 Human UniProt ID Q12834
Background	Cyclins, regulatory subunits which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
Synonyms	p55CDC
Reference Data

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