CD46 Mouse Monoclonal Antibody [Clone ID: 122.2]

Specifications

Product Data
Clone Name	122.2
Applications	FC, FN, IF, IHC, IP, WB
Recommend Dilution	Functional Assays (Neutralization): Use Azide free Antibody. Western Blot: 0.5-1 µg/ml. Flow Cytometry: 0.5-1 µg/106 cells. Immunoprecipitation: 1-2 µg/500 µg protein lysate. Immunofluorescence: 1-2 µg/ml. Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT. Recommended Positive Control: HeLa, K-562 or MOLT-4 cells, Kidney.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human CD46 protein.
Specificity	This antibody recognizes Human CD46. Other Species not tested. Cellular Localization: Cell surface.
Isotype	IgG1
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	56-66 kDa
Gene Name	CD46 molecule
Database Link	Entrez Gene 4179 Human UniProt ID P15529
Background	CD46 acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. CD46 acts as a co-stimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.
Synonyms	TLX, MIC10
Reference Data

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