c-Myb (MYB) Mouse Monoclonal Antibody [Clone ID: MYB286]

Specifications

Product Data
Clone Name	MYB286
Applications	FC, IF, IP, WB
Recommend Dilution	ELISA: Use BSA free Antibody for coating. Flow Cytometry: 0.5-1 μg/million cells. Immunofluorescence: 1-2 μg/ml. Western Blotting: 0.5-1 μg/ml. Immunoprecipitation: 1-2 μg/500 μg protein lysate. Positive Control: Jurkat, MOLT-4, COLO or BT-20 cells. Colon or breast carcinomas.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic peptide, corresponding to aa 119-135 (RRKVEQEGYPQESSKAG) of c-Myb oncoprotein, coupled to KLH.
Specificity	Recognizes c-Myb Oncoprotein. Cellular Localization: Nuclear.
Isotype	IgG1
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	~75 kDa
Gene Name	MYB proto-oncogene, transcription factor
Database Link	Entrez Gene 4602 Human UniProt ID P10242
Background	The highly leukemogenic avian retrovirus E26 contains two oncogenes, v-Myb and v-Ets, which are expressed together as a fusion protein. The cellular homolog of v-Myb, designated c-Myb, encodes a transcription factor. Deletion or disruption of a negative regulatory domain mapping within the carboxy-ter- minal domain of c-Myb results in enhanced transactivating capacity and in parallel, leads to activation of its ability to transform hemopoietic cells. c-Myb is expressed prefere ntially, but not exclusively, in immature hemopoietic cells and its expression decreases as cells differentiate.
Synonyms	c-Myb
Reference Data

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