Dibromo-Tyrosine (DiBrY) Mouse Monoclonal Antibody [Clone ID: 3A5]

Specifications

Product Data
Clone Name	3A5
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA (Ref.1). Western blot.  immunohistochemistry on Paraffin Sections: 10 μg/ml (Ref.1).
Host	Mouse
Clonality	Monoclonal
Immunogen	3,5-Dibromo-Tyrosine KLH-conjugated.
Specificity	Specific for di-halogenated Tyrosine. Recognizes Dibromo-Tyrosine (DiBrY), N-acetylated dichlorotyrosine (NAcDiClY), diiodo-tyrosine (DiIY), N-bromo-succinimide-treated BSA, HOCl-treated BSA and dibromo-tyrosine conjugated BSA. Bromotyrosine (BrY) is weakly recognized. There was no cross reactivity obtained with dityrosine-conjugated BSA, peroxynitrite-treated BSA, tyrosine (Y), chlorotyrosine(ClY), 3-3’-dityrosine (DiY), 3-aminotyrosine (NH2Y), 3-nitrotyrosine (NO2Y), phenylalanine (Phe), o-phosphotyrosine, 3, 4-dihydroxyphenylacetic acid (DOPA) and 2-hydroxy-4-phenylacetic acid. No cross reactivity was obtained with brominated nucleic acids 5-bromouracil, 5-bromouridine, and 8-bromoguanosine.
Isotype	IgG1
Formulation	10 mM PBS pH 7.4 State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in 200 µl aqua bidest to 0.1 mg/ml
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Synonyms	Diamine oxidase, AOC1, DAO1, Amiloride-binding protein, Histaminase, Kidney amine oxidase
Reference Data

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