HLA Class II DR Mouse Monoclonal Antibody [Clone ID: B-C10]

Specifications

Product Data
Clone Name	B-C10
Applications	FC
Recommend Dilution	Flow Cytometry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	RPMI 8866 and DAUDI cell lines. Myeloma X63/AG.8653 x Balb/c spleen cells.
Specificity	This Monoclonal antibody recognises the HLA Class II (DR) membrane.
Isotype	IgG2b
Formulation	PBS without carriers and preservatives State: Azide Free State: Liquid purified (0.2 μm sterile filtered) IgG fraction
Concentration	lot specific
Purification	Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Background	Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC II alpha/beta dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain (beta-2-Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles.
Synonyms	HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR
Reference Data

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