p13 Myeloid Marker Mouse Monoclonal Antibody [Clone ID: BM-3]

Specifications

Product Data
Clone Name	BM-3
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA: 1/5,000-1/20,000. Western Blot: 1/500-1/5,000. Immunoprecipitation: 1/100. Flow Cytometry. Immunohistochemistry on Frozen Sections: (1/100). The antibody does not stain tissue after paraffin treatment.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A BALB/c mouse was immunized with nuclei from Pokeweed mitogen stimulated Human peripheral blood lymphocytes.
Specificity	This purified Mouse monoclonal antibody reacts specifically with p13 in Human granulocytes and monocytes residing in lymphoid and non-lymphoid tissues. The antibody recognizes a 13 kDa band corresponding to p13. Cross reactivity from other sources has not been determined.
Isotype	IgG1
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Background	p13 is a specific marker for human myeloid cells. Induction studies using HL-60 cells show that p13 is expressed during the early phases of myeloid differentiation. The mAb anti-p13 recognizes a nuclear antigen expressed in human granulocytes (98%) and monocytes (80%) residing in lymphoid and non-lymphoid tissues. BM3 is an early marker of myeloid differentiation. Immunoprecipitation experiments using S35 methionine labeled human myeloid leukemia cells show that BM3 identifies a 13,000 Dalton protein.
Reference Data

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