Pneumocystis carinii Mouse Monoclonal Antibody [Clone ID: 3F6]

Specifications

Product Data
Clone Name	3F6
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. Recommended Dilution: 1/10-1/50. Positive Control: Infected Tissue. Preparation and Pretreatment 1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C. 2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) technique in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	Anti-Pneumocystis carinii antibody reacts with an epitope on the organism which is resistant to Formalin, Picric Acid, Paraffin, as well as Alchohol and Xylene. No cross-reactivity has been demonstrated with other fungi or parasitic organisms. Cellular Localization: Membranous
Isotype	IgM
Formulation	PBS, pH 7.4 State: Supernatant State: Liquid Tissue Culture Supernatant Stabilizer: 0.9% BSA Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Pneumocystis carinii is a fungal organism which is detected in Human tissues (typically in lung in immunocompromised patients) in the trophozoite stage.
Reference Data

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